Molecular analysis of the interaction between olea europaea and the biotrophic fungus spilocaea oleagina. Feb 10, 2014 palestine technical university research journal. Pdf relative susceptibility of new olive cultivars to. In both experiments, 1 week before the inoculation with v. V, covering fundamental and applied aspects of plant pathology. Lesion expansion was affected p spilocaea oleagina or showing disorders caused by. Oct 10, 2017 we have developed a visionbased program to detect symptoms of olive quick decline syndrome oqds on leaves of olea europaea l. The disease affects trees throughout the growing season and can cause significant losses in yield. Anatomical observations on sound leaves and by spilocaea oleagina cast. Climatic factors influence the emergence and reemergence of infectious diseases by shifting the geographic range of the insect vector and lengthening the reproductive cycles of insects. Pdf olive leaf spot or peacock spot, caused by the fungal pathogen spilocaea oleagina, can cause reduced growth and yield in olive trees olea. Characterisation of the main palestinian olea europaea l.
The tree is distinctly important in the economic and social life of the people. If the sexual stage exists and is discovered, it will belong to the genus venturia. Neoram wg je kontaktni, preventivni fungicid na osnovi 37,5% bakrenog oksiklorida. Conidia used in this evaluation were obtained from naturally infected olive leaves in. The journal of plant pathology jpp is the international journal of the italian phytopathological society s. Cycloconium oleagina causes the olive leaf spot ols or the pea cock eye disease on olives where ever the trees are grown gonzalezlamothe et al. Cultivated crops in palestine varies according to different geomorphology and.
The mycelium typically develops on the leaf tissue. Spilocaea oleagina downy mildew pseudoperonospora humuli less copper, more efficiency. Specific primers 18sf forward and 18sr reverse were used to confirm the identity of s. Olive leaves with obvious ols symptoms were cut into 5 2 cm segments, placed in a tube containing 5 20ml sterile distilled water and shaken vigorously. Centennial olive trees as a reservoir of genetic diversity. A dna marker specific for resistance to spilocaea oleagina.
Pdf survey of spilocaea oleagina, causal agent of olive. The digital repository of palestine technical university. Resistance of olivetree to spilocaea oleagina is mediated by the synthesis of phenolic compounds. Timing of fungicide applications and their high costs and possible effects on human health and the environment are critical for an effective control. It helps windows select the right program to open the file. Cookies are small text files that contain a string of characters and uniquely identifies a browser. Briefly, the plant material was digested in nitric acid 33% under high pressure in a microwave. A preflowering treatment was only satisfactory in the case of bordeaux. Trends in breeding new olive varieties in israel for quality. Article full text enhanced pdf format, 180766 bytes article sharing. Cycloconium oleagineum is the causal agent of olive olea europaea scab.
Olive cultivar and maturation process on the oviposition. The research results indicate that the majority of olive growers are not implemented to date protection against pathogens of peacock olive leaf spot. Spilocaea oleaginea is a deuteromycete fungal plant pathogen, the cause of the disease olive peacock spot, also known as olive leaf spot and birds eye spot. Relative susceptibility of new olive cultivars to spilocaea oleagina. Pdf spilocaea oleagina in olive groves of southern spain. Phylogeny of the fungus spilocaea oleagina, the causal. Olive leaf spot ols disease or peacock disease is caused by the fungus spilocaea oleagina, it is the most destructive disease on olive trees in most regions of palestine. Phylogeny of the fungus spilocaea oleagina, the causal agent of. Application of leaf extracts of ambrosia artemisiifolia to control. Effectsoftemperatureinoculumconcentrationleafageandcontinuousandinterruptedwetnessoninfectionofoliveplantsby spilocaea oleagina.
Genetic variation in spilocaea oleagina populations from new. The chemicals applied to olive trees were zineb with or without cu qxychloride, tetrachloroethylthiotetrahydrophthalimide. The journals mission is the promotion of plant health for mediterranean crops, climate and regions, safe food production, and the transfer of new knowledge on plant diseases and their sustainable management. This disease is widespread in many olivegrowing regions of the world trapero casas et al. University of california, division of agriculture and natural. An assessment of peacock olive leaf spot spilocaea. Evaluation of the incidence and severity of olive leaf spot. Olive cultivar and maturation process on the oviposition preference of bactrocera oleae rossi diptera. The effect of local fungicides on conidial germination of spilocaea oleagina in palestine. For the resistant variety, these symptoms are composed of small necrotic lesions, whereas for the susceptible variety, they appear as extended necrotic spots. Each was applied on 23 may before flowering, on 23 may and 5 july, or on 23 may and 23 september. Overall, tetraconazole reduced the disease and, as a systemic action, may be associated with dodine or copper to control spilocaea oleagina particularly when the disease is well developed. Pdf resistance of olive tree to spilocaea oleagina is mediated. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs.
Arbequina i18 is more tolerant than the other two cultivars to frost, while koroneiki i38 is the most sensitive. Pdf survey of spilocaea oleagina, causal agent of olive leaf spot. In order to get insight, an in vitro culture of the fungus has been established and its identity confirmed by amplified fragment length polymorphism analysis. Files are available under licenses specified on their description page. Olive leaf spot ols is found in many parts of the world and cause readuced growth and yield in olive trees. Formuliran je u obliku vodotopivih granula, proizvedenih novom fluid bed tehnologijom, koja osigurava bolju topivost u vodi, vecu iskoristivost, te bolju pokrovnost i ucinkovitost. Resistance of olive tree to spilocaea oleagina is mediated by the. This habit has been associated with a defence reaction of the host involving mobilization and breakdown of the phenolic glucoside oleuropin and inhibition of pectolytic enzymes produced by the pathogen. Losses arise mostly from defoliation of severely infected trees, with consequently reduced yield. Osser vazioni anatomiche su foglie di olivo sane ed. Full length article resistance of olive tree to spilocaea. The effect of local fungicides on conidial germination of. In addition, in 2002, a severe pruning was made in january, which probably caused an imbalance between vegetative and.
Sporulation on olive leaf spot lesions followed similar trends over the same temperatures and moisture conditions, with maximum sporulation at 15c and 100% rh but none at 25c and 70% rh. Epidemiology of leaf spot of olive tree caused by spilocaea oleagina in southern spain article pdf available in acta horticulturae 474474. Genetic variation in spilocaea oleagina populations from. An assessment of peacock olive leaf spot spilocaea oleagina.
All structured data from the file and property namespaces is available under the creative commons cc0 license. The disease is controlled by application of coppercontaining fungicides prior to winter rains. Pre harvest interval 14 days for hop, potato and tomato outdoor and in the greenhouse, 28 days for grapevine, for orchards is ensured with the time of use. The fungus spilocaea oleagina causes peacock leaf spot in olive. Effectsoftemperatureinoculumconcentrationleafageandcontinuousandinterruptedwetnessoninfectionofoliveplantsbyspilocaeaoleagina. Download csv file this work is licensed under a creative commons attributionnoncommercialsharealike 3. Phylogenyofthefungus spilocaea oleagina,thecausalagentof. Annual and cumulative yields of irta olive clones planted in 1998 tarragona, catalonia. All structured data from the file and property namespaces is available under the creative commons. Evaluation of the incidence and severity of olive leaf. Among nonpathogen taxa, aureobasidium pullulans, the species complex of cladosporium cladosporioides and devriesia spp. Global warming and its effect on dermatology and plants. In this study, investigations were carried out to measure the incidence % infected leaves and severity number of lesionsleaf of ols in olive growing regions in palestine, including the regions of hebron, bethlehem, tulkarm, salfit, jenin, nablus, ramallah and qalqilyah. Phytopathologia mediterranea is an international journal edited by the mediterranean phytopathological union.
Olive leaf spot caused by the fungus, spilocaea oleagina, is the most important leaf disease of olives in many olivegrowing regions worldwide with yield losses of up to 20%. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf file directly to your computer, from where it. Palestine, spilocaea oleagina, peacock spot, disease severity, olea europaea. Previous work predicted disease from leaf images with deep learning but required a vast amount of data which was obtained via crowd sourcing such as the plantvillage. Effects of temperature, inoculum concentration, leaf age, and. Pdf resistance of olive tree to spilocaea oleagina is. Trends in breeding new olive varieties in israel for. Fusicladium oleagineum, the fungal causal agent of olive. Symptoms are mainly confined to leaves and appear as dark brown, circular, zonate spots surrounded by yellow haloes peacocks eye. Low yields were recorded in both years, particularly in 2003, likely because a severe shoot defoliation occurred in all treatments at the end of the spring, caused by an infection of spilocaea oleagina, the olive leaf spot. Global warming or climate tropicalization is the cause of increasing temperature and humidity in the temperate areas of europe and north america. A much lower relative frequency was revealed for spilocaea oleagina and for other putative pathogens including fusarium spp. Survey of spilocaea oleagina, causal agent of olive leaf spot, in north of iran article pdf available march 2011 with 486 reads how we measure reads.
Of the total number of sampled locations, only twelve of them had percentage of infection lower than 10% which is the threshold tolerance spilocaea oleagina castagne hughes fungus infection. Guideline for the biological evaluation of fungicides spilocaea oleagina olive leaf spot 1. Spilocaea oleagina induces a foliar necrosis whose speed of onset and expansion distinguishes clearly the two studied varieties according to their behaviour to the leafspot disease. Pdf evaluation of the incidence and severity of olive. In this study, investigations were carried out to measure the incidence % infected leaves and severity number of lesionsleaf of ols in olive growing regions in palestine, including the regions of hebron, bethlehem, tulkarm, salfit, jenin, nablus, ramallah and qalqiyah. Leccino not receptive anatomical differences of the leaf as a whole and. Taxonomyofthegenusventuria the genus venturia belongs to the phylum ascomycota, class dothideomycetes schoch et al. Pdf epidemiology of leaf spot of olive tree caused by.
Viruega jr, trapero a 1999 epidemiology of leaf spot of olive tree caused by spilocaea oleagina in southern spain. Spilocaea oleagina is a deuteromycete because it has no known sexual stage. Lesions can be seen on the upper surface of leaves. Use the link below to share a fulltext version of this article with your friends and colleagues. Spilocaea oleagina in olive groves of southern spain aps journals. Olive leaf spot ols caused by the fungus spilocaea oleagina cast. Resistance of olive tree to spilocaea oleagina is mediated by the synthesis of phenolic compounds article pdf available in international journal of agriculture and biology january 2010 with.
Article full text enhanced pdf format, 303501 bytes. This plant disease commonly affects the leaves of olive trees worldwide. Silicon reduces the incidence of venturia oleaginea. Biologyandepidemiologyof venturia speciesaffectingfruit. The effects of temperature, relative humidity rh, leaf wetness and leaf age on conidium germination were investigated for spilocaea oleagina, the causal organism of olive leaf spot. Pdf olive scab caused by the mitosporic fungus spilocaea oleagina is the most important foliar disease of olive. Mediterranean clonal selections evaluated for modern hedgerow. Apr 18, 2019 specific primers 18sf forward and 18sr reverse were used to confirm the identity of s. Morphological and histochemical investigation of the response of olea europaea leaves to fungal attack by spilocaea oleagina.
Relative susceptibility of new olive cultivars to spilocaea oleagina, colletotrichum acutatum, and pseudocercospora cladosporioides. The author has studied the anatomical characters comparatively between two cv. Box pcr using the box repair primer was used to discriminate between isolates. Studies on controlling spilocaea oleagina cycloconium. International journal of scientific research in science and technology. Silicon reduces the incidence of venturia oleaginea castagne. A file extension is the characters after the last dot in a file name.
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